Thermophilus muts
WebbGregory Armstrong, in Comprehensive Natural Products Chemistry, 1999. 2.12.4.3.4 Thermus thermophilus. Thermus thermophilus, an aerobic thermophilic bacterium … WebbThe Tth MutS DNA mismatch protein recognizes heteroduplex DNAs containing mispaired or unpaired bases. This 91.8-kDa protein binds in vitro to heteroduplex DNAs containing …
Thermophilus muts
Did you know?
Webb10 sep. 2009 · We previously reported the construction of a plasmid vector carrying mutS-lacZ, a fusion gene designed to obtain a bifunctional chimeric protein consisting of MutS (mismatch binding protein) and β-galactosidase (Sachadyn et al., 2007).The fusion gene was created by in-frame cloning of E. coli lacZ gene into an expression plasmid carrying … Webb10 jan. 2007 · T.thermophilus is a Gram-negative eubacterium that can grow at temperatures greater than 75°C ( 17 ). In general, proteins isolated from T.thermophilus …
WebbWe previously established that Thermus thermophilus MutS2 (ttMutS2) possesses endonuclease activity. In this study, we report that a Smr-deleted ttMutS2 mutant retains … Webb1 mars 1996 · Recognition of mispaired or unpaired bases during DNA mismatch repair is carried out by the MutS protein family. Here, we describe the isolation and characterization of a thermostable MutS homolog from Thermus aquaticus YT-1. Sequencing of the mutS gene predicts an 89.3-kDa polypeptide sharing extensive amino acid sequence homology …
All living organisms have DNA repair systems to counteract DNA damage caused by sunlight, chemical agents and DNA replication errors (1). DNA repair … Visa mer Webb25 maj 2001 · T. thermophilus HB8 MutS protein was purified as described (Takamatsu et al., 1996). A mutant protein K597A, where the lysine residue at position 597 is replaced by alanine, was constructed using the polymerase chain reaction. DNA manipulations were performed using standard procedures (Sambrook et al., 1989).
WebbThermus thermophilus MutS, a thermostable mismatch-recognizing protein, is utilized in PCR to suppress nonspecific amplification by preventing synthesis from mismatched …
Webb1 sep. 1998 · Thermus thermophilus MutS (1 µM) was denatured with the indicated concentrations of GdnHCl and the spectrum of each reaction mixture in a 5 × 5 mm cell … chime bell station aiken scWebb30 maj 2016 · Further, we observed that under the conditions of experiment, MutS from D. radiodurans showed a higher affinity towards mismatched DNA than those two others (Fig. 2a, b). In the presence of ADP, the specificity of MutS from T. thermophilus strongly increased (Fig. 3b) to exceed even that of MutS from D. radiodurans (Fig. 2c). grading sheet for teachers printableWebb1 sep. 2016 · Further, we observed that under the conditions of experiment, MutS from D. radiodurans showed a higher affinity towards mismatched DNA than those two others (Fig. 2 a, b). In the presence of ADP, the specificity of MutS from T. thermophilus strongly increased (Fig. 3 b) to exceed even that of MutS from D. radiodurans (Fig. 2 c). chime bells jean shepherdWebb21 juli 2013 · Thermus thermophilus MutS, a thermostable mismatch-recognizing protein, is utilized in PCR to suppress nonspecific amplification by preventing synthesis from mismatched primers. T. thermophilus RecA also decreases nonspecific amplification by promoting proper hybridization between the primer and template. chime betaWebbThe mutS gene, implicated in DNA mismatch repair, was cloned from an extremely thermophilic bacterium, Thermus thermophilus HB8, and its nucleotide sequence encoded a 819-amino acid protein with a molecular mass of 91.4 kDa, suggesting that T.thermophilus MutS protein was a tetramer in its native state. The mutS gene, … chime better business bureauWebb28 apr. 2011 · T. thermophilus HB8 strains lacking mutM (Δ mutM) and ttha0675 (Δ ttha0675) were constructed by substituting the target gene with the thermostable kanamycin-resistance gene, HTK [34], through homologous recombination as previously described [35], [36]. grading sheet in filipinoWebb29 dec. 2000 · T. thermophilus MutS protein is stable from pH 1.5 to 12 at 25 °C and at a neutral pH up to 80 °C (19). Results from protein denaturation and limited proteolysis … chime billing statement